Extraction of Catechins from Aegle marmelos Fruit Pulp: Statistical Optimization Using Response Surface Methodology and Artificial Neural Networks

Aegle marmelos is a medicinal herb that has variety of biological constituents. “Catechins” are class phenolic compounds have therapeutic value. The present study employs batch extraction with methanol as solvent to extract the catechins from pulp fruit. Box–Behnken design response surface methodology (RSM-BBD) used optimize operational parameters impacting catechin extraction, such concentration, pH, and time. Extraction 96.5% was achieved at concentration 80%, pH 6.24, soaking period 44.7 hrs (desirability: 0.966). Additionally, MATLAB’s artificial neural network (ANN) accurately estimate yield. antimicrobial activity methanolic tested against five different pathogens, including Streptococcus, Bacillus mega, Pseudomonas putida, cerecus, Staphylococcus aureus, using agar diffusion method tube dilution method. cereus, pseudomonas putida showed high activity.